Water Samples — Microbiome Insights

Water samples often contain a high diversity of microbes, including bacteria or archaea. Our team has experience working with various microbes from filtered water samples. We have provided a protocol prepared by Dr. Steven Hallam’s lab that outlines small volume filtration of water samples. This protocol is outlined for seawater samples but can be used for any type of water sample.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3148634/

Services we offer:

iTag Amplicon Sequencing of 16S, 18S, ITS2 and archaeal V4-V5
qPCR (quantitative PCR) for total bacterial content
Shotgun Metagenomic Sequencing
Bacterial Whole Genome Sequencing (coming soon)

Collection

Basic instructions:

Step 1
Prepare your samples using the water filtration procedure outlined in the link above. For your convenience, these steps are demonstrated in a video as well as in written format.
Step 2
Store the filters at -80 until ready to be shipped.

Protocol

Water samples are extracted using the MoBio Powersoil Extraction kit, optimized for extraction using the ThermoFisher KingFisher automated robot. A bead beating step is included in the protocol for increased lysis.

We optimize the amount of starting material used, to improve sequencing yields in low biomass samples while ensuring sample integrity and minimizing cross-contamination by processing these sample types under laminar flow.

Resources

Video from Hallam lab: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3148634/
Shipping Instructions

We use Microbiome Insights because they offer unbiased, expedited, professional services at a reasonable cost and this has streamlined our research. Their expertise in the microbiome gives us confidence in our results and their bioinformatic and statistical support provides us with a comprehensive understanding of the results.

Katherine Cook

Assistant Professor at Wake Forest University

Microbiome Insights

5.0

2018-10-25T12:28:50+00:00

Katherine Cook

Assistant Professor at Wake Forest University

We use Microbiome Insights because they offer unbiased, expedited, professional services at a reasonable cost and this has streamlined our research. Their expertise in the microbiome gives us confidence in our results and their bioinformatic and statistical support provides us with a comprehensive understanding of the results.

https://microbiomeinsights.com/testimonials/2381/

From point of initial contact to final bioinformatic report, I was thoroughly pleased with the amplicon sequencing and bioinformatics services provided by Microbiome Insights. Detailed instructions facilitated the cross-border shipment of my samples to the Microbiome Insights facility; turnaround time for the extraction, sequencing and bioinformatics analysis was excellent.

Irene Yang, PhD, RN
Assistant Professor at Emory University

Microbiome Insights

5.0

2018-11-22T17:03:57+00:00

Irene Yang, PhD, RN
Assistant Professor at Emory University

From point of initial contact to final bioinformatic report, I was thoroughly pleased with the amplicon sequencing and bioinformatics services provided by Microbiome Insights. Detailed instructions facilitated the cross-border shipment of my samples to the Microbiome Insights facility; turnaround time for the extraction, sequencing and bioinformatics analysis was excellent.

https://microbiomeinsights.com/testimonials/2551/

When I was working on a project to characterize the microbiome of freshwater sponges, I turned to Microbiome Insights for my sequencing needs because they offered timely and cost-effective services for both my sponge and water samples. Just a few weeks after submitting my samples I received high-quality sequences that have now become the backbone of my graduate research.

Scott Sugden
Graduate Student at the University of Alberta.

Microbiome Insights

5.0

2018-11-22T17:04:49+00:00

Scott Sugden
Graduate Student at the University of Alberta.

When I was working on a project to characterize the microbiome of freshwater sponges, I turned to Microbiome Insights for my sequencing needs because they offered timely and cost-effective services for both my sponge and water samples. Just a few weeks after submitting my samples I received high-quality sequences that have now become the backbone of my graduate research.

https://microbiomeinsights.com/testimonials/2553/

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