Over the past several years the Microbiome Insights team has invested in the development of new tools and techniques for obtaining high-quality, actionable skin microbiome data for our partners and clients in the cosmetics and dermatology industry.
When designing a new skin microbiome study, we always have an important discussion: which variable region should be sequenced? Although many assume that, for characterizing skin bacteria, primers targeting regions V1-3 are superior to those targeting the V4 region, it’s not so straightforward.
All current primers have their limitations—namely, that they underestimate the abundance of some skin-dwelling bacteria, poorly capturing skin commensals.
Our team members Pedro Dimitriu and Hilary Leung redesigned the V4 primer pair under the direction of Microbiome Insights co-founder Dr. Bill Mohn, and found that the new primers resulted in the detection of more bacterial genera, while improving error rates. The new primer also addressed a main limitation of common primers used for the v4 region: it can detect Propionibacterium acnes—the most abundant human skin bacterium.
Thus, we are now pleased to offer our clients this exclusive V4_skin primer in order to help them make the most of their skin microbiome surveys.
https://microbiomeinsights.com/wp-content/uploads/2018/11/logo-new-1030x999.png00Karl Moranhttps://microbiomeinsights.com/wp-content/uploads/2018/11/logo-new-1030x999.pngKarl Moran2018-08-13 18:21:072018-08-21 06:26:49Better skin microbiome analyses using new 16S V4 region primers developed by Microbiome Insights’ scientific team
Microbiome data is leading to innovative solutions in diverse industries, from human and animal health to agriculture and the built environment. Next-generation sequencing has allowed researchers new insights into the microbial world with high levels of resolution—that is, they can precisely identify many of the bacteria and other microorganisms present. Not only that, but these technologies have enabled higher throughput than ever before. Foundational technologies, such as amplification and sequencing of phylogenetic markers, including the 16S rRNA gene, have become standard tools for understanding how microbial communities are structured and how they respond to changes in their environment.
However, amplicon sequencing does have some limitations in the type and resolution of the information it provides. This is where metagenomics — the direct recovery of total genomic information from the environment — can make a difference. Amplicon sequencing readily provides information at roughly the genus level; with care, it can identify microbial species and strains only under specific circumstances. Metagenomics reliably provides up to strain-level resolution (Figure 1). It also provides information about function—what the microorganisms’ genes equip them to do.
Figure 1. Species level classification of Staphylococcus species in skin samples recovered from amplicon sequencing and metagenomics. Metagenomics was able to resolve the taxonomy up to species and show that different body types select for different Staphylococcus species. Data from patient HV07 from Oh et al. 2014 (doi: 10.1038/nature13786).
Functional information is useful to understand the mechanisms underlying the changes in the microbial community, to reconstruct the metabolism of the community as an entity, and to discover new genes and pathways (Figure 2). The addition of functional information is also helpful to understand what groups provide what functions and how much redundancy exists for that function, which can have implications for the degree of resilience of the community (how it can bounce back after perturbations).
Figure 2. A. Changes in the abundances of key carbohydrate active enzymes in the soil ten years after forest harvesting. Differences were present in enzymes involved in the degradation of plant carbohydrates such as cellulose and hemicellulose. Modified from Cardenas et al. 2014 (doi:10.1038/ismej.2015.57) B. Metabolic reconstruction of the aerobic n-alkane degradation by partially-recovered genome from a metagenome of an oil reservoir. Expression levels are represented in blue barplots. Modified from Liu 2018 (DOI 10.1186/s40168-017-0392-1)
A second advantage of metagenomics is that it recovers data from all microbial community members, so the information will not be limited to bacteria (as when using a 16S rRNA) but also include data for fungi, viruses, and other groups. One example: using metagenomics, Oh et al. 2014 (Figure 3) mapped the abundance of bacterial and fungal species, and viral groups to different skin locations, identified functional gene differences across sites, and recovered 67 partial genomes (bacterial, viral, and eukaryotic). When samples have low diversity (e.g. enrichments), metagenomics can recover high quality draft genome sequences from community members. The genome of Kuenenia stuttgardiensis, one of the first characterized anaerobic ammonia oxidizers, was obtained from a metagenome of a bioreactor sample (see doi:10.1038/nature04647) without the need for cultivation.
Figure 3. (A) Average multi-kingdom relative abundances for 15 healthy adults stratified by skin characteristics. (B) Detailed phyla-level composition for two of those patients. Data from Oh et al. 2014 (doi: 10.1038/nature13786).
Metagenomics also comes with its own limitations. Since sequencing is done for the whole community, analysis can be challenging if too much host DNA is present or for samples with very low biomass. In the first case, most of the data will be of little interest since the host is not the target. In the second case, only a small part of the community will be reflected in the data, leading to a biased understanding of the microbiome. Finally, the applications of metagenomics depend on the depth of sequencing (Figure 4). Having higher sequencing coverage allows for recovery of data from more community members, assembly of short reads into larger contigs, and the use of those contigs to reconstruct genes, pathways, and genomes.
Figure 4. Effect of sequencing effort on the range of possible analyses of metagenomes.
In addition to these challenges, the public databases which are used for data comparison are constrained. These databases contain sequence information as well additional data such as the organism the sequences came from, the location and date of sampling, functional annotation, and links to related publications. Databases link sequence information with taxonomy and function and represent the historic efforts of researchers worldwide (and consequently their biases). These databases are limited first because most genes in any genome, even those from well-studied groups, lack biochemical characterization; and second, databases are biased towards human-related and pathogenic groups. Poorly represented groups in the databases include the archaea, fungi, viruses, and small eukaryotes; poorly represented environments include soils. Yet, this may not be a roadblock, but a challenge that will lead us to a better understanding of the microbial world.
“Both the cost and complexity barriers to metagenomic and metatranscriptomic sequencing have been greatly reduced, meaning these shotgun approaches are now practical ways to very precisely profile the human microbiome and other microbial communities,” says Curtis Huttenhower, Microbiome Insights Scientific Advisory Board member and Associate Professor of computation biology and bioinformatics at the Harvard T.H. Chan School of Public Health (Boston). “Metagenomics can now easily provide strain tracking and functional information that is difficult to obtain using amplicon sequencing, and these can further be integrated with metatranscriptomics, metabolomics, or other culture-independent molecular data to understand microbial community bioactivity.”
Microbiome Insights provides a full suite of services, including both amplicon sequencing and metagenomics. We can help you answer the question: where will metagenomics can take you?
https://microbiomeinsights.com/wp-content/uploads/2018/02/827581062.jpg10801920Erick Cardenas Poirehttps://microbiomeinsights.com/wp-content/uploads/2018/11/logo-new-1030x999.pngErick Cardenas Poire2018-03-28 20:44:212018-12-17 21:02:26What can metagenomics do for you?